A shot there is same like a shot to any other part of the body, but because of nerves, it’s gonna hurt. Unlike most other parts of your body, though, your scrotum lacks protection in the form of bones, large muscle mass, and fat.
It's not only about the shot, any sensation in that place is extra ordinary, and generate the flow quickly to brain, that's why it is so painfull in shot and so good in sex.
WHY IS THAT IMPORTANT PLACE UNPROTECTED
It's all because of the sperm. The testes’ job is to produce sperm, and sperm are very fragile. They’re extremely sensitive to high and low temperatures, and must be kept away from the rest of the body and relatively exposed to maintain the right climate. They can handle human body temps for only one to four hours, or the average amount of time it takes them to travel through the female reproductive tract and fertilize an egg. Internal testes or any type of significant shielding for them would heat them up too much, too early and make them drop out of the race well before reaching the egg, rendering them useless.
The scrotum isn’t just a dumb sack swaying in the breeze. In deference to our genetic interests, our bodies subconsciously thermoregulate our balls by flexing the cremasteric muscle and drawing the scrotum up closer to the body when it gets too cold and dropping it when it’s hot. This optimized, on-the-fly sperm storage is precise enough that each testicle can be repositioned independent of its twin in order to get the temperature just right, explaining their sometimes asymmetrical dangle.
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